Logo der Universität Wien



67. Matveenko, M., Cichero, E., Fossa, P., Becker, C.F. (2016) Impaired Chaperone Activity of Human Heat Shock Protein Hsp27 Site-Specifically Modified with Argpyrimidine Angew. Chem. Intl. Ed., 55, 11397-11402.

66. Koehbach, J., Gruber, C., Becker, C.F., Kreil, D., Jilek, A., (2016) A MALDI TOF/TOF based approach for the identification of D-amino acids in biologically active peptides and proteins. J. Proteome Res., 15, 1487–1496.

65. Conibear, A.C., Farbiarz, K., Mayer, R.L., Matveenko, M., Kählig, H., Becker, C.F. (2016) Arginine side-chain modification that occurs during copper-catalysed azide–alkyne click reactions resembles an advanced glycation end product. Org. Biomol. Chem.,14, 6205-6211.

64. Aladini, F., Araman, C., Becker, C.F. (2016) Chemical Synthesis and Characterization of Elastin-Like Polypeptides (ELPs) with Variable Guest Residues. J. Pep. Sci., 22, 334–342.

63. Martelli,T., Ravera, E., Louka, A., Cerofolini, L., Hafner, M., Fragai, M., Becker, C.F., Luchinat, C. (2016) Atomic-Level Quality Assessment of Enzymes Encapsulated in Bioinspired Silica. Chem. Eur.J., 22, 425–432.

Book chapters:

“Semisynthesis of Membrane-Attached Proteins Using Split Inteins” (2016) Hackl, S., Schmid, A., Becker, C.F. in Split Inteins, Methods in Molecular Biology, Ed. H. Mootz, Humana Press (DOI: 10.1007/978-1-4939-6451-2)

“Chemoselective attachment of lipids to proteins” (2016) Becker, C.F. in Chemoselective and Biorthogonal Ligation Reactions: Concepts and Applications, Ed. R. Algar, I. Melnitz, P. Dawson, John Wiley & Sons


62. Lechner, C., Becker, C.F. (2015) Silaffins in Silica Biomineralization and Biomimetic Silica Precipitation, Mar. Drugs, 13, 5297-5333.

61. Bello, C., Wang, S., Meng, L., Moremen, K.W., Becker, C.F. (2015) A PEGylated Photocleavable Auxiliary Mediates the Sequential Enzymatic Glycosylation and Native Chemical Ligation of Peptides, Angew. Chem. Intl. Ed., 54, 7711-7715.

60. Bello, C., Kikul, F., Becker, C.F. (2015) Efficient generation of peptides hydrazides via direct hydrazinolysis of Peptidyl-Wang-TentaGel resins, J. Pep. Sci., 21, 201–207.

59. Lechner, C., Becker, C.F. (2015) Immobilizing proteins on silica with site-specifically attached modified silaffin peptides, Biomater. Sci., 3, 288-297.


58. Chu, N. K., Shabbir, W., Bove-Fenderson, E., Araman, C., Lemmens-Gruber, R., Harris, D.A., Becker, C.F. (2014) A C-terminal membrane enchors affects affects the interactions of prion proteins with lipid membranes, J. Biol. Chem, 289, 30144–30160.

57. Bello, C., Farbiarz, K., Möller, J.F., Becker, C.F., Schwientek, T. (2014) A quantitative and site-specific chemoenzymatic glycosylation approach for PEGylated MUC1 peptides, Chem. Sci., 5, 1634-1641.

56. Schwaighofer, A., Kotlowski, C., Araman, C., Chu, N., Mastrogiacomo, R., Becker, C.F., Pelosi, P., Knoll, W., Larisika, M., Nowak, C. (2013) Honey bee odorant-binding protein 14: effects on  thermal stability upon odorant binding revealed by FT-IR spectroscopy and CD measurements, Eur. Biophys J., 43,105-112.

55. Tripsianes, K., Chu, N.K., Friberg, A., Sattler, M., Becker, C.F. (2013) Studying Weak and Dynamic Interactions of Posttranslationally Modified Proteins using Expressed Protein Ligation, ACS Chem. Biol., 9, 347-352.

54. Lechner, C., Becker, C.F. (2014) A sequence-function analysis of the silica precipitating silaffine R5 peptide, J. Pep. Sci., 20, 152-158.


53. Rauh, D., Fischer, F., Gertz, M., Lakshminarasimhan, M., Bergbrede, T., Aladini, F., Kambach, C., Becker, C.F., Zerweck, J., Schutkowski, M., Steegborn, C. (2013) An acetylome peptide microarray reveals specificities and deacetylation substrates for all human Sirtuin isoforms, Nat. Comm.

52. Lechner, C.C., Becker C.F. (2013) Modified silaffin R5 peptides enable encapsulation and release of cargo molecules from biomimetic silica particles, Bioorg. Med. Chem., 21,3533-3541.

51. Chu, N.K., Becker, C.F. (2013) Recombinant expression of soluble murine Prion protein for C-terminal modification. FEBS Letters, 587, 430-435.

50. Marcinowski, M., Seitz, C., Rosam, M., Elferich, J., Behnke, J., Bello, C., Feige, M.J., Becker, C.F., Antes, I., Buchner, J. (2013) Conformational Selection in Substrate Recognition by Hsp70 Chaperones. J. Mol. Biol., 425, 466-474.

49. Gogolin, L., Schroeder, H., Itzen, A., Goody, R.S., Niemeyer, C.M., Becker, C.F. (2012) Protein-DNA Arrays as Tools for Detection of Protein-Protein Interactions by Mass Spectrometry. ChemBioChem, 14, 92-99.


48. Gertz, M., Nguyen, G., Fischer, F., Sünkel, B., Schlicker, C., Fraenzel, B., Tomaschewski, J., Aladini, F., Becker, C.F., Wolters, D., Steegborn, C. (2012) A molecular mechanism for direct Sirtuin activation by resveratrol. PLOS ONE, 7, e49761.

47. Stein, M., Beck, P., Kaiser, M., Dudler, R., Becker, C.F., Groll, M. (2012) One-shot NMR analysis of microbial secretions identifies highly potent proteasome inhibitor. Proc. Natl. Acad. Sci. U.S.A., 18367-18371.

46. Lechner, C.C., Becker C.F. (2012) Exploring the effect of native and artificial peptide modifications on silaffin induced silica precipitation. Chem. Sci., 2012, 3 (12), 3500 - 3504.

45. Hong, G.S., Chen, C.P., Lin, M.H., Krüger, J., Becker, C.F., Fink, R.A., Fischer, W.B. (2012) Molecular dynamics simulations and conductance studies of the2 interaction of VP1 from Polio  virus and gp41 fusion peptide from 3 HIV-1 with lipid membranes. Mol. Membr. Biol., 29, 9-25.


44. Brehs, M., Bäuml, K., Becker, C.F. (2011) Chemical Synthesis of an Integral Membrane Enzyme – The Challenges of Diacylglycerol Kinase. Isr. J. Chem., 51, 930-936.

43.  Lahiri, S., Brehs, M., Olschewski, D., Becker, C.F. (2011) Total Chemical Synthesis of an Integral Membrane Enzyme: Diacylglycerol Kinase from Escherichia coli. Angew. Chem. Int. Ed.., 50, 3988-3992.

42. Weber, M., Prodöh, A., Dreher, C., Becker, C.F., Underhaug, J., Svane, A.S., Malmendal, A., Nielsen, NC., Otzen, D., Schneider, D. (2011) SDS-Facilitated In vitro Formation of a Transmembrane B-Type Cytochrome Is Mediated by Changes in Local pH. J. Mol. Biol., 407, 594–606.

41. Becker, C.F. (2011) Ambiguous Origin: Two Sides of an Ephrin Receptor Tyrosine Kinase. Chem. Biol., 18, 279-281.

40. Becker, C.F. (2011) Proteinsemisynthese – ein Werkzeug für komplex modifizierte Proteine, BIOspektrum, 3, 291.


39. Lahiri, S. Seidel, R., Engelhard, M., Becker, C.F. (2010) Photocontrol of STAT6 dimerization and translocation. Mol. BioSys., 6, 2423-2429.

38. Chu, N.K., Olschewski, D., Seidel, R., Winklhofer, K.F., Tatzelt, J., Engelhard, M., Becker, C.F. (2010) Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing. J. Pept. Sci., 16, 582–588.

37. Becker, C.F. (2010) Size Matters: Side-Chain Length Affects SH2 Substrate Binding. Chem. Biol., accepted.

36. Gogolin, L., Seidel, R., Engelhard, M., Goody, R.S., Becker, C.F. (2010) Semisynthesis of Human Thymidine Monophosphate Kinase. Biopolymers-Peptide Science, accepted.

35. Gerlach, H., Laumann, V., Martens, S., Becker, C.F., Goody, R.S., Geyer, M. (2009) HIV-1 Nef membrane association depends on charge, curvature, composition and sequence. Nat. Chem. Biol., 6, 46-53.

34. Schumacher, M.C., Resenberger, U., Seidel, R.P., Becker, C.F., Winklhofer, K.F., Oesterhelt, D., Tatzelt, J., Engelhard, M. (2010) Synthesis of a GPI Anchor Module Suitable for Protein Post-Translational Modification” Biopolymers, DOI 10.1002/bip.21380.


33. Chu, K.N., Becker C.F. (2009) Semisynthesis of membrane-Attached Prion Proteins. Methods in Enzymology, 462, 177-193.

32. Becker, C.F. (2009) Native Chemical Ligation – A Tool for Chemical Protein Synthesis. Chemical Biology (Wiley-VCH), 145-156.

31. Olschewski, D., Seidel, R., Tatzelt, J., Engelhard, M., Becker, C.F. (2009) Semisynthesis of Membrane-Attached Prion Proteins. Proceedings 30th European Peptide Symposium, 10-12.

Book review: “Manufacturing of Pharmaceutical Proteins” by Stefan Behme (2009), ChemBioChem.


30. Becker, C.F., Liu, X., Olschewski, D., Castelli, R., Seidel, R., Seeberger, P.H. (2008) Semisynthesis of a Glycosylphosphatidylinositol-Anchored Prion Protein. Angew. Chem. Intl. Ed. Engl., 47, 8215-8219.

29. Filchtinski, D., Bee, C., Savopol, T., Engelhard, M., Becker, C.F., Herrmann, C. (2008) Probing Ras Effector Interactions on Nanoparticle Supported Lipid Bilayers. Bioconj. Chem.,19, 1938-1944.

28. Rambold, A.S., Miesbauer, M., Olschewski, D., Seidel, R., Riemer, C., Smale, L., Brumm, L., Levy,M., Gazit, E., Oesterhelt, D., Baier, M., Becker, C.F., Engelhard, M., Winklhofer, K.F., Tatzelt, J. (2008) Green tea extracts interfere with the stress-protective activity of PrPC and the formation of PrPSc. J. Neurochem., 107, 218-229.

27. Schlicker, C., Gertz, M., Papatheodorou, P., Kachholz, B., Becker, C.F., Steegborn, C. (2008) Substrates and regulation mechanisms for the human mitochondrial Sirtuins Sirt3 and Sirt5. J. Mol. Biol., 382, 790-801.

26. Govindaraju, T., Jonkheijm, P., Gogolin, L., Schroeder, H., Becker, C.F., Niemeyer, C.M., Waldmann, H. (2008) Surface immobilization of biomolecules by click sulfonamide reaction. Chem. Com., 28, 3723-3725.

25. Olschewski, D., Becker, C.F. (2008) Chemical Synthesis and Semisynthesis of Membrane Protein. Mol. BioSyst., 4, 733–740.

24. Mili , J., Seidel, R., Becker, C.F., Goody, R.S., Engelhard, M. (2008) Semisynthesis of H-Ras with a glutamic acid methylester at position 61. Peptide Science, 90, 399-405.

23. Becker, C.F., Goody, R.S. (2008) Synthetic Peptides and Proteins to Elucidate Biological Function. Wiley Encyclopedia of Chemical Biology, Ed. Begley, T.P., John Wiley & Sons.

Book review: “Peptides from A to Z: A Concise Encyclopedia” by H.-D. Jakubke, N. Sewald (2008), ChemBioChem, 9, 2163.


22. Olschewski, D., Seidel, R., Miesbauer, M., Rambold, A.S., Oesterhelt, D., Winklhofer, K.F., Tatzelt, J., Engelhard, M., Becker, C.F. (2007) Semisynthetic murine Prion protein equipped with a GPI anchor mimic incorporates into cellular membranes. Chem. Biol., 14, 994-1006.

21. Schroeder, H., Ellinger, B., Becker, C.F., Waldmann, H., Niemeyer, C.M. (2007) Generation of Live Cell Microarrays by Means of DNA-Directed Immobilization of Specific Cell Surface Ligands. Angew. Chem. Int. Ed Engl., 46, 4180-4183.

20. Becker, C.F., Marsac, Y., Hazarika, P., Moser, J., Goody, R.S., Niemeyer, C.M. (2007) Functional Immobilization of the Small GTPase Rab6A on DNA-Gold-Nanoparticles Using a Site-Specifically Attached Polyethylene Glycol Linker and Thiol Place Exchange Reaction. ChemBioChem, 8, 32-36.


19. Marsac, Y., Cramer, J., Olschewski, D., Alexandrov, K., Becker, C.F. (2006) Site-specific attachment of polyethylene glycol-like oligomers to proteins and peptides. Bioconj. Chem., 17, 1492-1498.

18. Becker, C.F., Wacker, R., Bouschen, W., Seidel, R., Kolaric, B., Lang, P., Schroeder, H., Niemeyer, C.M., Spengler, B., Goody, R.S., Engelhard, M. (2006) Protein Arrays as Tools for Detection of Protein-Protein Interactions by Mass Spectrometry. Proceedings of the 19th Symposium of the American Peptide Society “Understanding biology using peptides”, San Diego, USA, June 18-23, 2005, Editor Sylvie E. Blondelle.

17. Volkmer, T., Becker, C.F., Prodöhl, A., Dreher, C., Finger, C., Schneider, D. (2006) Assembly of a transmembrane b-Type cytochrome is mainly driven by transmembrane helix interactions. BBA-Biomembranes, 1758, 1815-1822.

16. Kushnir, S., Marsac, Y., Breitling, R., Granovsky, I., Brok-Volchanskaya, V.,
Goody, R.S., Becker, C.F., Alexandrov, K. (2006) Rapid Production of Functionalized Recombinant Proteins: Marrying Ligation Independent Cloning and in Vitro Protein Ligation. Bioconj. Chem., 17, 610-617.

15. Becker, C.F., Seidel, R., Jahnz, M., Bacia, K., Niederhausen, T., Alexandrov, K., Schwille, P., Goody, R.S., Engelhard, M. (2006) C-terminal Fluorescence Labeling of Proteins for Interaction Studies on the Single Molecule Level. ChemBioChem, 7, 891-895.


14. Becker,C.F., Wacker,R., Bouschen,W., Seidel,R., Kolaric,B., Lang,P., Schroeder,H., Muller,O., Niemeyer,C.M., Spengler,B., Goody,R.S., and Engelhard,M. (2005) Direct Readout of Protein-Protein Interactions by Mass Spectrometry from Protein-DNA Microarrays. Angew. Chem. Int. Ed Engl., 44, 7635-7639.

13. Durek,T. and Becker,C.F. (2005) Protein semi-synthesis: New proteins for functional and structural studies. Biomol. Eng, 22, 153-172.

12. Becker,C.F., Lausecker,K., Balog,M., Kalai,T., Hideg,K., Steinhoff,H.J., and Engelhard,M. (2005) Incorporation of spin-labelled amino acids into proteins. Magn Reson. Chem., 43, S34-S39.

11. Eberth,A., Dvorsky,R., Becker,C.F., Beste,A., Goody,R.S., and Ahmadian,M.R. (2005) Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins. Biol. Chem., 386, 1105-1114.

10. Kochendoerfer,G.G., Clayton,D., and Becker,C.F. (2005) Chemical Synthesis Approaches to the Engineering of Ion Channels. Prot. Pept. Lett., 12, 737-741.

9. Kochendoerfer,G., Becker,C.F., Strop,P., Bass,R.B., Hansen,K.C., Locher,K., Ren,G., Yeager,M., and Rees,D.C. (2005) Conversion of a mechanosensitive channel protein from a membrane-embedded to a water-soluble form by covalent modification with amphiphiles. Biophys. J., 88, 333A.


8. Becker, C.F., Strop, P., Bass, R.B., Hansen, K.C., Locher, K.P., Ren, G., Yeager, M., Rees, D.C., Kochendoerfer, G.G. (2004) Conversion of a Mechanosensitive Channel Protein from a Membrane-embedded to a Water-soluble Form by Covalent Modification with Amphiphiles. J. Mol. Biol. 343, 747-758.

7. Becker, C.F., Clayton, D., Shapovalov, G., Lester, H.A. and Kochendoerfer, G.G. (2004) On-Resin Assembly of a Linkerless Lanthanoide(III)-Based Lumiescence Label and Its Application to the Total Synthesis of Site-Specifically Labeled Mechanosensitive Channels. Bioconj. Chem. 15, 1118-1124.

6. Becker, C.F., Oblatt-Montal, M., Kochendoerfer, G.G. and Montal, M. (2004) Chemical synthesis and single channel properties of tetrameric and pentameric TASPs (template-assembled synthetic proteins) derived from the transmembrane domain of HIV virus protein u (Vpu). J. Biol. Chem. 279, 17483-17489


5. Becker, C.F., Hunter, C.L., Seidel, R., Kent, S.B.H., Goody, R.S. and Engelhard, M. (2003) Total chemical synthesis of a functional interacting protein pair: The protooncogene H-Ras and the Ras-binding domain of its effector c-Raf1. Proc. Nat. Acad. Sci. U.S.A. 100, 5075-5080.


4. Becker, C.F., Hunter, C.L., Seidel, R.P., Kent, S.B., Goody, R.S. and Engelhard, M. (2001) A sensitive fluorescence monitor for the detection of activated Ras: total chemical synthesis of site-specifically labeled Ras binding domain of c-Raf1 immobilized on a surface. Chem. Biol. 8, 243-252.

3. Becker,C.F., Hunter,C.L., Seidel,R.P., Kent,S.B.H., Goody,R.S., and Engelhard,M. (2001) Synthesis of the Ras-RBD protein pair. In Lebl,M. and Houghten,R.H. (Eds.), Peptides: The wave of the future, American Peptide Society, pp. 870-872.

2. Gruen, M., Becker, C., Beste, A., Reinstein, J., Scheidig, A. and Goody, R.S. (1999) “2`Halo-ATP and –GTP analogues: Rational phasing tools for protein crystallography.” Protein Science 8, 2524-2528.

1. Gruen, M., Becker, C., Beste, A., Siethoff, C., Scheidig, A. and Goody, R.S. (1999) “Synthesis of 2’-Iodo- and 2’-Bromo-ATP and –GTP analogues as potential Phasing Tools for X-Ray Crystallography.” Nucleosides & Nucleotides 18 (1), 137-151.


University of Vienna
Biological Chemistry
Prof. Dr. Christian Becker
Währinger Straße 38
1090 Vienna

Office hours (Michaela Cherrier, Room 2H43):
Mon, Wed - Fri: 9 - 12 am, Thursday also 1.30 - 3.30 pm

T: +43-1-4277-705 01
University of Vienna | Universitätsring 1 | 1010 Vienna | T +43-1-4277-0