FWF funded Special Research Program "Targeted Protein Degradation"

Tailor-made ubiquitin chains for targeting the UPS or autophagy


Access to homogeneous, ubiquitinated proteins provides a unique tool in deciphering the complex ubiquitin-proteasome system (UPS) and the autophagy-lysosomal pathway (ALP), as the exact molecular composition of ubiquitin-tags is critical for recognition by parts of the UPS and ALP. To provide such tools, we will generate a set of modified Ub building blocks via protein semisynthesis by combining solid phase peptide synthesis (SPPS) and expression of Ub-intein fusion constructs in E. coli.



Assembly of homo- or heterotypic ubiquitin chains of different but fully controlled length using chemoselective ligation reactions.